› 8gca

X-ray diffraction
1.7Å resolution

Mouse acidic mammalian chitinase, catalytic domain in complex with N,N',N''-triacetylchitotriose at pH 4.74

Released:
DOI: 10.2210/pdb8gca/pdb
Model geometry
Fit model/data
PDB entry 8gca coloured by chain, front view.
PDB entry 8gca coloured by chain, side view.
PDB entry 8gca coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural characterization of ligand binding and pH-specific enzymatic activity of mouse Acidic Mammalian Chitinase.
Díaz RE, Ecker AK, Correy GJ, Asthana P, Young ID, Faust B, Thompson MC, Seiple IB, Van Dyken SJ, Locksley RM, Fraser JS
OpenAccess logo bioRxiv (2023)
PMID: 37398339

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187586 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Acidic mammalian chitinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 397 amino acids
Theoretical weight: 44.62 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q91XA9 (Residues: 1-391; Coverage: 82%)
Gene names: Chia, Chia1
Sequence domains: Glycosyl hydrolases family 18

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21212
Unit cell:
a: 76.066Å b: 91.719Å c: 106.132Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.196
Expression system: Cricetulus griseus

Quick links

Citations

2 mentions without citation

Structural characterization of ligand binding and pH-specific enzymatic activity of mouse Acidic Mammalian Chitinase.
Díaz et al. (2024)
1 more