› 8gqw

X-ray diffraction
2.48Å resolution

The Crystal Structures of a Swine SLA-2*HB01 Molecules Complexed with a CTL epitope from Asia1 serotype of Foot-and-mouth disease virus

Released:
DOI: 10.2210/pdb8gqw/pdb
PDB entry 8gqw coloured by chain, front view.
PDB entry 8gqw coloured by chain, side view.
PDB entry 8gqw coloured by chain, top view.
Source organisms:
Primary publication:
The Parallel Presentation of Two Functional CTL Epitopes Derived from the O and Asia 1 Serotypes of Foot-and-Mouth Disease Virus and Swine SLA-2*HB01: Implications for Universal Vaccine Development.
Feng L, Gao YY, Sun M, Li ZB, Zhang Q, Yang J, Qiao C, Jin H, Feng HS, Xian YH, Qi J, Gao GF, Liu WJ, Gao FS
Cells 11 (2022)
PMID: 36552780

Function and Biology Details

Reactions catalysed: 
Autocatalytically cleaves itself from the polyprotein of the foot-and-mouth disease virus by hydrolysis of a Lys-|-Gly bond, but then cleaves host cell initiation factor eIF-4G at bonds -Gly-|-Arg- and -Lys-|-Arg-.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-222960 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 275 amino acids
Theoretical weight: 31.75 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: E3WHS2 (Residues: 25-299; Coverage: 81%)
Gene name: SLA-2
Sequence domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 98 amino acids
Theoretical weight: 11.43 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07717 (Residues: 21-118; Coverage: 100%)
Gene name: B2M
Sequence domains: Immunoglobulin C1-set domain
Hu64 Chains: C, F
Molecule details ›
Chains: C, F
Length: 9 amino acids
Theoretical weight: 1.07 KDa
Source organism: Foot-and-mouth disease virus
Expression system: Not provided
UniProt:
  • Canonical: Q80B22 (Residues: 788-796; Coverage: 0%)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 48.626Å b: 98.458Å c: 166.233Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.213 0.26
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 mention without citation

The Parallel Presentation of Two Functional CTL Epitopes Derived from the O and Asia 1 Serotypes of Foot-and-Mouth Disease Virus and Swine SLA-2*HB01: Implications for Universal Vaccine Development.
Feng et al. (2022)