› 8h5j

X-ray diffraction
1.4Å resolution

Crystal structure of PETase S121E/A180V/P181V/D186H/N233C/S242T/N246D/S282C mutant from Ideonella sakaiensis

Released:
DOI: 10.2210/pdb8h5j/pdb
PDB entry 8h5j coloured by chain, front view.
PDB entry 8h5j coloured by chain, side view.
PDB entry 8h5j coloured by chain, top view.
Source organism: Ideonella sakaiensis
Entry authors: Lee SH, Seo H, Kim K-J

Function and Biology Details

Reaction catalysed: 
(Ethylene terephthalate)(n) + H(2)O = (ethylene terephthalate)(n-1) + ethylene terephthalate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102331 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(ethylene terephthalate) hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 300 amino acids
Theoretical weight: 31.61 KDa
Source organism: Ideonella sakaiensis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0K8P6T7 (Residues: 34-290; Coverage: 98%)
Gene name: ISF6_4831
Sequence domains: Dienelactone hydrolase family

Ligands and Environments

3 bound ligands:
Ligand K 3 x K
Ligand PO4 3 x PO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: C2
Unit cell:
a: 139.218Å b: 50.665Å c: 80.854Å
α: 90° β: 124.64° γ: 90°
R-values:
R R work R free
0.158 0.156 0.19
Expression system: Escherichia coli

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