› 8hgw

X-ray diffraction
2.8Å resolution

Crystal structure of MehpH in complex with MBP

Released:
DOI: 10.2210/pdb8hgw/pdb
PDB entry 8hgw coloured by chain, front view.
PDB entry 8hgw coloured by chain, side view.
PDB entry 8hgw coloured by chain, top view.
Source organism: Gordonia sp. P8219
Primary publication:
Molecular insights into the catalytic mechanism of plasticizer degradation by a monoalkyl phthalate hydrolase.
Chen Y, Wang Y, Xu Y, Sun J, Yang L, Feng C, Wang J, Zhou Y, Zhang ZM, Wang Y
Commun Chem 6 45 (2023)
PMID: 36859434

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173728 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 289 amino acids
Theoretical weight: 31.5 KDa
Source organism: Gordonia sp. P8219
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2MHH5 (Residues: 24-311; Coverage: 93%)
Gene name: mehpH
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

2 bound ligands:
Ligand 1BO 1 x 1BO
Ligand PHT 4 x PHT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CAMD BEAMLINE GCPCC
Unit cell:
a: 76.95Å b: 48.95Å c: 306.325Å
α: 90° β: 93.73° γ: 90°
R-values:
R R work R free
0.229 0.228 0.26
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

A Novel and Efficient Phthalate Hydrolase from Acinetobacter sp. LUNF3: Molecular Cloning, Characterization and Catalytic Mechanism.
Fan et al. (2023)
1 more

1 mention without citation

Molecular insights into the catalytic mechanism of plasticizer degradation by a monoalkyl phthalate hydrolase.
Chen et al. (2023)