› 8hid

X-ray diffraction
2.2Å resolution

HUMAN ERYTHROCYTE CATALSE COMPLEXED WITH BT-Br

Released:
DOI: 10.2210/pdb8hid/pdb
Model geometry
Fit model/data
PDB entry 8hid coloured by chain, front view.
PDB entry 8hid coloured by chain, side view.
PDB entry 8hid coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A catalase inhibitor: Targeting the NADPH-binding site for castration-resistant prostate cancer therapy.
Cao YY, Chen YY, Wang MS, Tong JJ, Xu M, Zhao C, Lin HY, Mei LC, Dong J, Zhang WL, Qin YX, Huang W, Zhang D, Yang GF
OpenAccess logo Redox Biol 63 102751 (2023)
PMID: 37216701

Function and Biology Details

Reaction catalysed: 
2 H(2)O(2) = O(2) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137332 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catalase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 527 amino acids
Theoretical weight: 59.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P04040 (Residues: 1-527; Coverage: 100%)
Gene name: CAT
Sequence domains:

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM
1 bound ligand:
Ligand LLR 4 x LLR
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 83.466Å b: 140.871Å c: 233.428Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.225 0.265
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

1 review citation

The mechanism of ferroptosis and its related diseases.
Feng et al. (2023)

1 mention without citation

A catalase inhibitor: Targeting the NADPH-binding site for castration-resistant prostate cancer therapy.
Cao et al. (2023)