› 8hrp

X-ray diffraction
1.99Å resolution

Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium glutamicum ATCC13032 in complex with NAD and G3P

Released:
DOI: 10.2210/pdb8hrp/pdb
Model geometry
Fit model/data
PDB entry 8hrp coloured by chain, front view.
PDB entry 8hrp coloured by chain, side view.
PDB entry 8hrp coloured by chain, top view.
Source organism: Corynebacterium glutamicum ATCC 13032
Primary publication:
Structure-Guided Protein Engineering of Glyceraldehyde-3-phosphate Dehydrogenase from Corynebacterium glutamicum for Dual NAD/NADP Cofactor Specificity.
Son HF, Yu H, Hong J, Lee D, Kim IK, Kim KJ
J Agric Food Chem 71 17852-17859 (2023)
PMID: 37935620

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236337 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 342 amino acids
Theoretical weight: 37.16 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01651 (Residues: 1-334; Coverage: 100%)
Gene names: Cgl1588, cg1791, gap
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAD 8 x NAD
4 bound ligands:
Ligand G3H 8 x G3H
Ligand GOL 14 x GOL
Ligand EDO 6 x EDO
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P21
Unit cell:
a: 86.946Å b: 119.849Å c: 140.632Å
α: 90° β: 91.27° γ: 90°
R-values:
R R work R free
0.166 0.164 0.201
Expression system: Escherichia coli

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