8i9t

Electron Microscopy
3.6Å resolution

Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State Dbp10-1

Released:

Function and Biology Details

Reactions catalysed:
ATP + H(2)O = ADP + phosphate
Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 55-mer (preferred)
PDBe Complex ID:
PDB-CPX-292627 (preferred)
Entry contents:
51 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (54 distinct):
Brix domain-containing protein Chain: CA
Molecule details ›
Chain: CA
Length: 316 amino acids
Theoretical weight: 36.48 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0RXZ3 (Residues: 1-316; Coverage: 100%)
Gene name: CTHT_0004820
Sequence domains: Brix domain
Ribosome biogenesis protein C8F11.04 Chain: CB
Molecule details ›
Chain: CB
Length: 391 amino acids
Theoretical weight: 43.7 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S7X0 (Residues: 1-391; Coverage: 100%)
Gene name: CTHT_0037280
Sequence domains: Ribosomal protein L1p/L10e family
Ribosome biogenesis protein ERB1 Chain: CC
ATP-dependent RNA helicase Chain: CE
Large ribosomal subunit protein uL10 Chain: CF
60S ribosome subunit biogenesis protein NIP7 Chain: CG
Nucleolar GTP-binding protein 1 Chain: CH
RRM domain-containing protein Chain: CI
Pescadillo homolog Chain: CJ
Ribosome biogenesis protein NSA2 homolog Chain: CK
CP-type G domain-containing protein Chain: CL
60S ribosomal protein l7-like protein Chains: CM, LF
Eukaryotic translation initiation factor 6 Chain: CN
DUF2423 domain-containing protein Chain: CO
Nucleolar protein 2 Chain: CP
Ribosome biogenesis protein RLP24 Chain: CQ
Nucleolar protein 16 Chain: CR
AdoMet-dependent rRNA methyltransferase SPB1 Chain: CS
rRNA processing protein Chain: CU
60S ribosomal subunit-like protein Chain: CX
rRNA-processing protein Chain: Cz
RNA helicase Chain: Cb
Ribosomal RNA-processing protein 15 Chain: Ch
Large ribosomal subunit protein uL3 Chain: LB
Large ribosomal subunit protein uL4 C-terminal domain-containing protein Chain: LC
60S ribosomal protein L6 Chain: LE
60S ribosomal protein L8 Chain: LG
Large ribosomal subunit protein uL6 alpha-beta domain-containing protein Chain: LH
60S ribosomal protein L12-like protein Chain: LK
60S ribosomal protein L13 Chain: LL
Large ribosomal subunit protein eL14 domain-containing protein Chain: LM
Ribosomal protein L15 Chain: LN
60S ribosomal protein L16-like protein Chain: LO
Large ribosomal subunit protein uL22 Chain: LP
Large ribosomal subunit protein uL15/eL18 domain-containing protein Chain: LQ
60S ribosomal protein L20 Chain: LS
60S ribosomal protein l21-like protein Chain: LT
60S ribosomal protein l23-like protein Chain: LV
Large ribosomal subunit protein uL23 N-terminal domain-containing protein Chain: LX
KOW domain-containing protein Chain: LY
60S ribosomal protein L32-like protein Chain: Le
60S ribosomal protein l33-like protein Chain: Lf
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 Chain: Lh
60S ribosomal protein L36 Chain: Li
Ribosomal protein L37 Chain: Lj
Ribosomal protein Chain: Lq
Ribosomal RNA-processing protein 1 Chain: Cc
Brix domain-containing protein Chain: Cd
Protein MAK16 Chain: Ce
60S ribosome biogenesis protein Rrp14 Chain: Cf
Brix domain-containing protein Chain: Cg
RNA (3341-MER) Chain: C1
RNA (256-MER) Chain: C2
RNA (161-MER) Chain: C3

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.6Å
Relevant EMDB volumes: EMD-35283