› 8ia3

X-ray diffraction
3.5Å resolution

Crystal structure of human USF2 bHLHLZ domain in complex with DNA

Released:
DOI: 10.2210/pdb8ia3/pdb
Model geometry
Fit model/data
PDB entry 8ia3 coloured by chain, front view.
PDB entry 8ia3 coloured by chain, side view.
PDB entry 8ia3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Tetramerization of upstream stimulating factor USF2 requires the elongated bent leucine zipper of the bHLH-LZ domain.
Huang C, Xia M, Qiao H, Liu Z, Lin Y, Sun H, Yu B, Fang P, Wang J
OpenAccess logo J Biol Chem 105240 (2023)
PMID: 37690682

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-270508 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Upstream stimulatory factor 2 Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 114 amino acids
Theoretical weight: 13.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15853 (Residues: 235-346; Coverage: 32%)
Gene names: BHLHB12, USF2
Sequence domains: Helix-loop-helix DNA-binding domain
DNA (5'-D(P*GP*AP*CP*GP*GP*GP*CP*AP*CP*GP*TP*GP*AP*CP*GP*CP*GP*C)-3') Chains: C, G
Molecule details ›
Chains: C, G
Length: 18 nucleotides
Theoretical weight: 5.57 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*GP*CP*GP*CP*GP*TP*CP*AP*CP*GP*TP*GP*CP*CP*CP*GP*TP*C)-3') Chains: D, H

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL19U1
Unit cell:
a: 78.847Å b: 56.951Å c: 232.118Å
α: 90° β: 90.92° γ: 90°
R-values:
R R work R free
0.245 0.242 0.297
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 mention without citation

Tetramerization of upstream stimulating factor USF2 requires the elongated bent leucine zipper of the bHLH-LZ domain.
Huang et al. (2023)