› 8ib4

Electron Microscopy
4.3Å resolution

Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature

Released:
DOI: 10.2210/pdb8ib4/pdb
PDB entry 8ib4 coloured by chain, front view.
PDB entry 8ib4 coloured by chain, side view.
PDB entry 8ib4 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis of respiratory complex adaptation to cold temperatures.
Shin YC, Latorre-Muro P, Djurabekova A, Zdorevskyi O, Bennett CF, Burger N, Song K, Xu C, Paulo JA, Gygi SP, Sharma V, Liao M, Puigserver P
Cell (2024)
PMID: 39395414
Related structures: EMD-35331

Function and Biology Details

Reactions catalysed: 
NADH + ubiquinone + 5 H(+)(Side 1) = NAD(+) + ubiquinol + 4 H(+)(Side 2)
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 66-mer (preferred)
PDBe Complex ID:
PDB-CPX-271248 (preferred)
Entry contents:
54 distinct polypeptide molecules
Macromolecules (54 distinct):
NADH-ubiquinone oxidoreductase chain 3 Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 13.22 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P03899 (Residues: 1-115; Coverage: 100%)
Gene names: Nd3, mt-Nd3
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase, chain 3
NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial Chain: B
Molecule details ›
Chain: B
Length: 224 amino acids
Theoretical weight: 24.72 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9DC70 (Residues: 1-224; Coverage: 100%)
Gene name: Ndufs7
Sequence domains: NADH ubiquinone oxidoreductase, 20 Kd subunit
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial Chain: C
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial Chain: D
NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial Chain: E
NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial Chain: F
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial Chain: G
NADH-ubiquinone oxidoreductase chain 1 Chain: H
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial Chain: I
NADH-ubiquinone oxidoreductase chain 6 Chain: J
NADH-ubiquinone oxidoreductase chain 4L Chain: K
NADH-ubiquinone oxidoreductase chain 5 Chain: L
NADH-ubiquinone oxidoreductase chain 4 Chain: M
NADH-ubiquinone oxidoreductase chain 2 Chain: N
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial Chain: O
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial Chain: P
NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial Chain: Q
NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial Chain: R
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 Chain: S
Acyl carrier protein, mitochondrial Chains: T, U
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 Chain: V
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 Chain: W
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 Chain: X
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 Chain: Y
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 Chain: Z
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 Chain: a
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 Chain: b
NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial Chain: c
NADH dehydrogenase [ubiquinone] 1 subunit C2 Chain: d
NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 Chain: e
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 Chain: f
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial Chain: g
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial Chain: h
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 Chain: i
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial Chain: j
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 Chain: k
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial Chain: l
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 Chain: m
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 Chain: n
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 Chain: o
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 Chain: p
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 Chain: q
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 Chain: r
NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial Chain: s
Cytochrome b-c1 complex subunit 1, mitochondrial Chains: AA, Aa
Cytochrome b-c1 complex subunit 2, mitochondrial Chains: AB, Ab
Cytochrome b Chains: AC, Ac
Cytochrome c1, heme protein, mitochondrial Chains: AD, Ad
Cytochrome b-c1 complex subunit Rieske, mitochondrial Chains: AE, AI, Ae
Cytochrome b-c1 complex subunit 7 Chains: AF, Af
Cytochrome b-c1 complex subunit 8 Chains: AG, Ag
Cytochrome b-c1 complex subunit 6, mitochondrial Chains: AH, Ah
Cytochrome b-c1 complex subunit 9 Chains: AJ, Aj
Cytochrome b-c1 complex subunit 10 Chains: AK, Ak

Ligands and Environments

17 bound ligands:
Ligand 3PE 20 x 3PE
Ligand SF4 6 x SF4
Ligand UQ1 1 x UQ1
Ligand PC1 3 x PC1
Ligand FES 2 x FES
Ligand FMN 1 x FMN
Ligand UQ9 1 x UQ9
Ligand CDL 9 x CDL
Ligand ADP 1 x ADP
Ligand NDP 1 x NDP
Ligand ZN 1 x ZN
Ligand EHZ 2 x EHZ
Ligand HEM 4 x HEM
Ligand U10 2 x U10
Ligand UQ6 2 x UQ6
Ligand HEC 2 x HEC
Ligand 3PH 2 x 3PH
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 4.3Å
Relevant EMDB volumes: EMD-35331

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