› 8id3

Electron Microscopy
3.1Å resolution

Cryo-EM structure of the 9-hydroxystearic acid bound GPR120-Gi complex

Released:
DOI: 10.2210/pdb8id3/pdb
PDB entry 8id3 coloured by chain, front view.
PDB entry 8id3 coloured by chain, side view.
PDB entry 8id3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Unsaturated bond recognition leads to biased signal in a fatty acid receptor.
Mao C, Xiao P, Tao XN, Qin J, He QT, Zhang C, Guo SC, Du YQ, Chen LN, Shen DD, Yang ZS, Zhang HQ, Huang SM, He YH, Cheng J, Zhong YN, Shang P, Chen J, Zhang DL, Wang QL, Liu MX, Li GY, Guo Y, Xu HE, Wang C, Zhang C, Feng S, Yu X, Zhang Y, Sun JP
Science eadd6220 (2023)
PMID: 36862765
Related structures: EMD-35356

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-279981 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 339 amino acids
Theoretical weight: 37.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
scFv16 Chain: S
Molecule details ›
Chain: S
Length: 285 amino acids
Theoretical weight: 30.36 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: Y
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Free fatty acid receptor 4 Chain: R

Ligands and Environments

1 bound ligand:
Ligand 7NR 1 x 7NR
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.1Å
Relevant EMDB volumes: EMD-35356
Expression system: Spodoptera frugiperda

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Citations

9 review citations

Signaling pathways in cancer metabolism: mechanisms and therapeutic targets.
You et al. (2023)
8 more