› 8idf

X-ray diffraction
3.7Å resolution

Crystal structure of human TUT1 complexed with U6 snRNA

Released:
DOI: 10.2210/pdb8idf/pdb
Model geometry
Fit model/data
PDB entry 8idf coloured by chain, front view.
PDB entry 8idf coloured by chain, side view.
PDB entry 8idf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanism of U6 snRNA oligouridylation by human TUT1.
Yamashita S, Tomita K
OpenAccess logo Nat Commun 14 4686 (2023)
PMID: 37563152

Function and Biology Details

Reactions catalysed: 
ATP + RNA(n) = diphosphate + RNA(n+1)
UTP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-275355 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Speckle targeted PIP5K1A-regulated poly(A) polymerase Chain: A
Molecule details ›
Chain: A
Length: 537 amino acids
Theoretical weight: 58.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H6E5 (Residues: 1-599; Coverage: 61%)
Gene names: RBM21, TUT1
Sequence domains:
RNA (53-MER) Chain: B
Molecule details ›
Chain: B
Length: 53 nucleotides
Theoretical weight: 17.01 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P6522
Unit cell:
a: 78.724Å b: 78.724Å c: 370.272Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.306 0.305 0.339
Expression systems:
  • Escherichia coli
  • Not provided

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