› 8ipc

X-ray diffraction
2.2Å resolution

The recombinant NZ-1 Fab complexed with the PDZ tandem fragment of A. aeolicus S2P homolog with the PA14 tag inserted between the residues 181 and 184

Released:
DOI: 10.2210/pdb8ipc/pdb
Model geometry
Fit model/data
PDB entry 8ipc coloured by chain, front view.
PDB entry 8ipc coloured by chain, side view.
PDB entry 8ipc coloured by chain, top view.
Source organisms:
Primary publication:
Recombinant production of antibody antigen-binding fragments with an N-terminal human growth hormone tag in mammalian cells.
Adachi Y, Kaneko MK, Kato Y, Nogi T
Protein Expr Purif 208-209 106289 (2023)
PMID: 37160213

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-239360 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
The recombinantly-expressed heavy chain of the monoclonal antibody NZ-1 Chain: H
Molecule details ›
Chain: H
Length: 221 amino acids
Theoretical weight: 23.55 KDa
Source organism: Rattus norvegicus
Expression system: Homo sapiens
The recombinantly-expressed light chain of the monoclonal antibody NZ-1 Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.35 KDa
Source organism: Rattus norvegicus
Expression system: Homo sapiens
Putative zinc metalloprotease aq_1964 Chain: A

Ligands and Environments

No bound ligands
2 modified residues:
Ligand PCA 1 x PCA
Ligand SNN 1 x SNN

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 51.935Å b: 75.086Å c: 173.522Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.243 0.27
Expression systems:
  • Homo sapiens
  • Escherichia coli

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