› 8k4l

X-ray diffraction
2.1Å resolution

Crystal structure of NRF1 homodimer in complex with DNA

Released:
DOI: 10.2210/pdb8k4l/pdb
Model geometry
Fit model/data
PDB entry 8k4l coloured by chain, front view.
PDB entry 8k4l coloured by chain, side view.
PDB entry 8k4l coloured by chain, top view.
Source organisms:
Primary publication:
Molecular mechanism of specific DNA sequence recognition by NRF1.
Liu K, Li W, Xiao Y, Lei M, Zhang M, Min J
OpenAccess logo Nucleic Acids Res (2023)
PMID: 38055835

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-270576 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Nuclear respiratory factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 232 amino acids
Theoretical weight: 25.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q16656 (Residues: 54-284; Coverage: 46%)
Gene name: NRF1
Sequence domains: NLS-binding and DNA-binding and dimerisation domains of Nrf1
DNA (14-MER) Chains: C, D
Molecule details ›
Chains: C, D
Length: 14 nucleotides
Theoretical weight: 4.28 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL02U1
Spacegroup: P212121
Unit cell:
a: 37.757Å b: 118.827Å c: 121.821Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.243
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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