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› 8kab

Electron Microscopy
3.3Å resolution

Mycobacterium smegmatis 50S ribosomal subunit-HflX complex

Released:
DOI: 10.2210/pdb8kab/pdb
PDB entry 8kab coloured by chain, front view.
PDB entry 8kab coloured by chain, side view.
PDB entry 8kab coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Cryo-EM structures reveal the molecular mechanism of HflX-mediated erythromycin resistance in mycobacteria.
Srinivasan K, Banerjee A, Sengupta J
Structure (2024)
PMID: 39029461
Related structures: EMD-37007

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 35-mer (preferred)
PDBe Complex ID:
PDB-CPX-300605 (preferred)
Entry contents:
33 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (35 distinct):
50S ribosomal protein bL37 Chain: 3
Molecule details ›
Chain: 3
Length: 24 amino acids
Theoretical weight: 2.84 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QTP4 (Residues: 1-24; Coverage: 100%)
Gene name: MSMEG_1916
Large ribosomal subunit protein uL2 Chain: C
Molecule details ›
Chain: C
Length: 278 amino acids
Theoretical weight: 30.43 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSD4 (Residues: 1-278; Coverage: 100%)
Gene names: MSMEG_1439, MSMEI_1403, rplB
Sequence domains:
Large ribosomal subunit protein uL3 Chain: D
Large ribosomal subunit protein uL4 Chain: E
Large ribosomal subunit protein uL5 Chain: F
Large ribosomal subunit protein uL6 Chain: G
Large ribosomal subunit protein bL9 Chain: H
Large ribosomal subunit protein uL10 Chain: I
Large ribosomal subunit protein uL11 Chain: J
Large ribosomal subunit protein uL13 Chain: K
Large ribosomal subunit protein uL14 Chain: L
Large ribosomal subunit protein uL15 Chain: M
Large ribosomal subunit protein uL16 Chain: N
Large ribosomal subunit protein bL17 Chain: O
Large ribosomal subunit protein uL18 Chain: P
Large ribosomal subunit protein bL19 Chain: Q
Large ribosomal subunit protein bL20 Chain: R
Large ribosomal subunit protein bL21 Chain: S
Large ribosomal subunit protein uL22 Chain: T
Large ribosomal subunit protein uL23 Chain: U
Large ribosomal subunit protein uL24 Chain: V
Large ribosomal subunit protein bL25 Chain: W
Large ribosomal subunit protein bL27 Chain: X
Large ribosomal subunit protein bL28 Chain: Y
Large ribosomal subunit protein uL29 Chain: Z
Large ribosomal subunit protein uL30 Chain: a
Large ribosomal subunit protein bL32 Chain: b
Large ribosomal subunit protein bL33A Chain: c
Large ribosomal subunit protein bL34 Chain: d
Large ribosomal subunit protein bL35 Chain: e
Large ribosomal subunit protein bL36 Chain: f
Large ribosomal subunit protein bL31 Chain: g
GTPase HflX Chain: h
23S ribosomal RNA Chain: A
5S ribosomal RNA Chain: B

Ligands and Environments

1 bound ligand:
Ligand GNP 1 x GNP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.3Å
Relevant EMDB volumes: EMD-37007
Expression system: Escherichia coli BL21

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