› 8khp

Electron Microscopy
3.67Å resolution

CULLIN3-KLHL22-RBX1 E3 ligase

Released:
DOI: 10.2210/pdb8khp/pdb
PDB entry 8khp coloured by chain, front view.
PDB entry 8khp coloured by chain, side view.
PDB entry 8khp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structure of the KLHL22 E3 ligase bound to an oligomeric metabolic enzyme.
Teng F, Wang Y, Liu M, Tian S, Stjepanovic G, Su MY
Structure (2023)
PMID: 37788672
Related structures: EMD-37247

Function and Biology Details

Reactions catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236512 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Kelch-like protein 22 Chains: A, B
Molecule details ›
Chains: A, B
Length: 634 amino acids
Theoretical weight: 71.74 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q53GT1 (Residues: 1-634; Coverage: 100%)
Gene name: KLHL22
Sequence domains:
Cullin-3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 768 amino acids
Theoretical weight: 89.06 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q13618 (Residues: 1-768; Coverage: 100%)
Gene names: CUL3, KIAA0617
Sequence domains:
E3 ubiquitin-protein ligase RBX1 Chains: E, F

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.67Å
Relevant EMDB volumes: EMD-37247
Expression system: Homo sapiens

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