› 8ojf

X-ray diffraction
3.04Å resolution

Arabidopsis thaliana Phosphoenolpyruvate carboxylase PPC1 with bound phosphate

Released:
DOI: 10.2210/pdb8ojf/pdb
PDB entry 8ojf coloured by chain, front view.
PDB entry 8ojf coloured by chain, side view.
PDB entry 8ojf coloured by chain, top view.
Source organism: Arabidopsis thaliana
Entry authors: Haesaerts S, Loris R, Larsen PB

Function and Biology Details

Reaction catalysed: 
Phosphate + oxaloacetate = H(2)O + phosphoenolpyruvate + HCO(3)(-)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-199956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate carboxylase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 974 amino acids
Theoretical weight: 111.4 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9MAH0 (Residues: 1-967; Coverage: 100%)
Gene names: At1g53310, F12M16.21, PPC1, p107
Sequence domains: Phosphoenolpyruvate carboxylase

Ligands and Environments

2 bound ligands:
Ligand PO4 3 x PO4
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21212
Unit cell:
a: 127.22Å b: 158.17Å c: 141.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.243
Expression system: Escherichia coli BL21(DE3)

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