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› 8onl

X-ray diffraction
1.9Å resolution

Crystal structure of D-amino acid aminotransferase from Aminobacterium colombiense point mutant E113A

Released:
DOI: 10.2210/pdb8onl/pdb
Model geometry
Fit model/data
PDB entry 8onl coloured by chain, front view.
PDB entry 8onl coloured by chain, side view.
PDB entry 8onl coloured by chain, top view.
Source organism: Aminobacterium colombiense
Primary publication:
In search for structural targets for engineering d-amino acid transaminase: modulation of pH optimum and substrate specificity.
Shilova SA, Matyuta IO, Khrenova MG, Nikolaeva AY, Klyachko NL, Minyaev ME, Khomutov AR, Boyko KM, Popov VO, Bezsudnova EY
Biochem J 480 1267-1284 (2023)
PMID: 37548495

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112690 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminotransferase class IV Chains: A, B
Molecule details ›
Chains: A, B
Length: 277 amino acids
Theoretical weight: 30.92 KDa
Source organism: Aminobacterium colombiense
Expression system: Escherichia coli
UniProt:
  • Canonical: D5EHC5 (Residues: 1-275; Coverage: 100%)
Gene name: Amico_1844
Sequence domains: Amino-transferase class IV

Ligands and Environments

1 bound ligand:
Ligand PLP 2 x PLP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: KURCHATOV SNC BEAMLINE K4.4
Spacegroup: P212121
Unit cell:
a: 61.429Å b: 88.013Å c: 99.772Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.213 0.263
Expression system: Escherichia coli

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Citations

1 citations in other articles

Expanded Substrate Specificity in D-Amino Acid Transaminases: A Case Study of Transaminase from Blastococcus saxobsidens.
Shilova et al. (2023)