› 8oo5

X-ray diffraction
2.25Å resolution

Crystal structure of human DCAF1 WD40 repeats (Q1250L) in complex with compound 13

Released:
DOI: 10.2210/pdb8oo5/pdb
PDB entry 8oo5 coloured by chain, front view.
PDB entry 8oo5 coloured by chain, side view.
PDB entry 8oo5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Reinstating targeted protein degradation with DCAF1 PROTACs in CRBN PROTAC resistant settings
Schroder M, Renatus M, Thoma CR
Biorxiv (2023)

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195228 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DDB1- and CUL4-associated factor 1 Chain: P
Molecule details ›
Chain: P
Length: 318 amino acids
Theoretical weight: 36.06 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9Y4B6 (Residues: 1079-1393; Coverage: 21%)
Gene names: DCAF1, KIAA0800, RIP, VPRBP

Ligands and Environments

2 bound ligands:
Ligand VUF 2 x VUF
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P6122
Unit cell:
a: 81.577Å b: 81.577Å c: 234.132Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.181 0.22
Expression system: Spodoptera frugiperda

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