› 8orl

X-ray diffraction
1.43Å resolution

Crystal structure of the S23226G missense variant of titin domain Fn3-56

Released:
DOI: 10.2210/pdb8orl/pdb
PDB entry 8orl coloured by chain, front view.
PDB entry 8orl coloured by chain, side view.
PDB entry 8orl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure determination and analysis of titin A-band fibronectin type III domains provides insights for disease-linked variants and protein oligomerisation.
Rees M, Nikoopour R, Alexandrovich A, Pfuhl M, Lopes LR, Akhtar MM, Syrris P, Elliott P, Carr-White G, Gautel M
J Struct Biol 215 108009 (2023)
PMID: 37549721

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186809 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Titin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 105 amino acids
Theoretical weight: 11.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8WZ42 (Residues: 21497-21598; Coverage: 0%)
Gene name: TTN
Sequence domains: Fibronectin type III domain

Ligands and Environments

2 bound ligands:
Ligand ZN 5 x ZN
Ligand CL 5 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2221
Unit cell:
a: 70.348Å b: 89.862Å c: 98.309Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.159 0.194
Expression system: Escherichia coli

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