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› 8ox8

Electron Microscopy
2.98Å resolution

Cryo-EM structure of ATP8B1-CDC50A in E2P autoinhibited "open" conformation

Released:
DOI: 10.2210/pdb8ox8/pdb
PDB entry 8ox8 coloured by chain, front view.
PDB entry 8ox8 coloured by chain, side view.
PDB entry 8ox8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Activation and substrate specificity of the human P4-ATPase ATP8B1.
Dieudonné T, Kümmerer F, Laursen MJ, Stock C, Flygaard RK, Khalid S, Lenoir G, Lyons JA, Lindorff-Larsen K, Nissen P
OpenAccess logo Nat Commun 14 7492 (2023)
PMID: 37980352
Related structures: EMD-17260

Function and Biology Details

Reaction catalysed: 
ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128957 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Phospholipid-transporting ATPase IC Chain: A
Molecule details ›
Chain: A
Length: 1251 amino acids
Theoretical weight: 143.98 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: O43520 (Residues: 1-1251; Coverage: 100%)
Gene names: ATP8B1, ATPIC, FIC1, PFIC
Sequence domains:
Cell cycle control protein 50A Chain: B
Molecule details ›
Chain: B
Length: 365 amino acids
Theoretical weight: 41.09 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q9NV96 (Residues: 1-361; Coverage: 100%)
Gene names: C6orf67, CDC50A, TMEM30A
Sequence domains: LEM3 (ligand-effect modulator 3) family / CDC50 family

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
3 bound ligands:
Ligand MG 1 x MG
Ligand IP9 1 x IP9
Ligand NAG 2 x NAG
1 modified residue:
Ligand PHD 1 x PHD

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.98Å
Relevant EMDB volumes: EMD-17260
Expression system: Saccharomyces cerevisiae

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