› 8psh

Solution NMR

HIGH RESOLUTION NMR STRUCTURE OF THE STEREOREGULAR (ALL-RP)-PHOSPHOROTHIOATE-DNA/RNA HYBRID D (G*PS*C*PS*G*PS*T*PS*C*PS*A*PS*G*PS*G)R(CCUGACGC), MINIMIZED AVERAGE STRUCTURE

Released:
DOI: 10.2210/pdb8psh/pdb
PDB entry 8psh coloured by chain, front view.
PDB entry 8psh coloured by chain, side view.
PDB entry 8psh coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Structure of a stereoregular phosphorothioate DNA/RNA duplex.
Bachelin M, Hessler G, Kurz G, Hacia JG, Dervan PB, Kessler H
Nat Struct Biol 5 271-6 (1998)
PMID: 9546216

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-122633 (preferred)
Entry contents:
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
DNA (5'-D(*DGP*(SC)P*(GS)P*(PST)P*(SC)P*(AS)P*(GS)P*(GS))-3') Chain: A
Molecule details ›
Chain: A
Length: 8 nucleotides
Theoretical weight: 2.58 KDa
Source organism: Synthetic construct
Expression system: Not provided
RNA (5'-R(*CP*CP*UP*GP*AP*CP*GP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.5 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
3 modified residues:
Ligand SC 2 x SC
Ligand PST 1 x PST
Ligand AS 1 x AS

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Not provided

Quick links

Citations

4 review citations

Structure and function of HIV-1 reverse transcriptase: molecular mechanisms of polymerization and inhibition.
Sarafianos et al. (2009)
3 more

1 mention without citation

Synthesis and properties of DNA oligomers containing stereopure phosphorothioate linkages and C-5 modified deoxyuridine derivatives.
Hara et al. (2020)