› 8qa4

Electron Microscopy
2.8Å resolution

MTHFR + SAH symmetric dis-inhibited state

Released:
DOI: 10.2210/pdb8qa4/pdb
PDB entry 8qa4 coloured by chain, front view.
PDB entry 8qa4 coloured by chain, side view.
PDB entry 8qa4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Dynamic inter-domain transformations mediate the allosteric regulation of human 5, 10-methylenetetrahydrofolate reductase.
Blomgren LKM, Huber M, Mackinnon SR, Bürer C, Baslé A, Yue WW, Froese DS, McCorvie TJ
OpenAccess logo Nat Commun 15 3248 (2024)
PMID: 38622112
Related structures: EMD-18298

Function and Biology Details

Reaction catalysed: 
5-methyltetrahydrofolate + NADP(+) = 5,10-methylenetetrahydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154911 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylenetetrahydrofolate reductase (NADPH) Chains: A, B
Molecule details ›
Chains: A, B
Length: 663 amino acids
Theoretical weight: 75.46 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P42898 (Residues: 1-656; Coverage: 100%)
Gene name: MTHFR
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand SAH 2 x SAH
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.8Å
Relevant EMDB volumes: EMD-18298
Expression system: Spodoptera frugiperda

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