› 8qfe

X-ray diffraction
1.5Å resolution

Cryogenic crystal structure of the Photoactivated Adenylate Cyclase OaPAC

Released:
DOI: 10.2210/pdb8qfe/pdb
Model geometry
Fit model/data
PDB entry 8qfe coloured by chain, front view.
PDB entry 8qfe coloured by chain, side view.
PDB entry 8qfe coloured by chain, top view.
Source organism: Oscillatoria acuminata PCC 6304
Primary publication:
Light-induced Trpin/Metout switching during BLUF domain activation in ATP-bound photoactivatable adenylate cyclase OaPAC.
Chretien A, Nagel MF, Botha S, de Wijn R, Brings L, Dörner K, Han H, Koliyadu JCP, Letrun R, Round A, Sato T, Schmidt C, Secareanu RC, von Stetten D, Vakili M, Wrona A, Bean R, Mancuso A, Schulz J, Pearson AR, Kottke T, Lorenzen K, Schubert R
J Mol Biol 168439 (2024)
PMID: 38185322

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-233846 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Family 3 adenylate cyclase Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 39.29 KDa
Source organism: Oscillatoria acuminata PCC 6304
Expression system: Escherichia coli
UniProt:
  • Canonical: K9TLZ5 (Residues: 1-350; Coverage: 96%)
Gene name: Oscil6304_3613
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand FMN 1 x FMN
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2221
Unit cell:
a: 52.856Å b: 146.202Å c: 103.595Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.189
Expression system: Escherichia coli

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Citations

1 citations in other articles

ATP-dependent conformational dynamics in a photoactivated adenylate cyclase revealed by fluorescence spectroscopy and small-angle X-ray scattering.
Ujfalusi-Pozsonyi et al. (2024)