› 8rnh

X-ray diffraction
1.6Å resolution

Crystal structure of HLA B*18:01 in complex with EEIEITTHF, an 9-mer epitope from Influenza A

Released:
DOI: 10.2210/pdb8rnh/pdb
Model geometry
Fit model/data
PDB entry 8rnh coloured by chain, front view.
PDB entry 8rnh coloured by chain, side view.
PDB entry 8rnh coloured by chain, top view.
Source organisms:
Primary publication:
Characterisation of novel influenza-derived HLA-B*18:01-restricted epitopes.
Leong SL, Murdolo L, Maddumage JC, Koutsakos M, Kedzierska K, Purcell AW, Gras S, Grant EJ
OpenAccess logo Clin Transl Immunology 13 e1509 (2024)
PMID: 38737448

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-274047 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: S6AU73 (Residues: 25-300; Coverage: 82%)
Gene name: HLA-B
Sequence domains:
RNA-directed RNA polymerase catalytic subunit Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.12 KDa
Source organism: Influenza A virus (A/Memphis/18/1978(H3N2))
Expression system: Not provided
UniProt:
  • Canonical: Q2VNC5 (Residues: 177-185; Coverage: 1%)
Gene name: PB1
Beta-2-microglobulin Chain: B

Ligands and Environments

2 bound ligands:
Ligand PEG 1 x PEG
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 50.75Å b: 81.627Å c: 110.556Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.202 0.234
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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