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› 8rqr

X-ray diffraction
2.19Å resolution

In meso structure of apolipoprotein N-acyltransferase, Lnt, from Escherichia coli in 7.10 monoacylglycerol

Released:
DOI: 10.2210/pdb8rqr/pdb
Model geometry
Fit model/data
PDB entry 8rqr coloured by chain, front view.
PDB entry 8rqr coloured by chain, side view.
PDB entry 8rqr coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
7.10 MAG. A Novel Host Monoacylglyceride for In Meso (Lipid Cubic Phase) Crystallization of Membrane Proteins.
Krawinski P, Smithers L, van Dalsen L, Boland C, Ostrovitsa N, Pérez J, Caffrey M
OpenAccess logo Cryst Growth Des 24 2985-3001 (2024)
PMID: 38585376

Function and Biology Details

Reaction catalysed: 
N-terminal S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[lipoprotein] +a glycerophospholipid = N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[lipoprotein] + a 2-acyl-sn-glycero-3-phospholipid + H(+).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150121 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apolipoprotein N-acyltransferase Chain: A
Molecule details ›
Chain: A
Length: 532 amino acids
Theoretical weight: 59.28 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P23930 (Residues: 1-512; Coverage: 100%)
Gene names: JW0654, b0657, cutE, lnt
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand A1H2K 8 x A1H2K
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I24
Unit cell:
a: 48.679Å b: 76.461Å c: 157.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.198 0.246
Expression system: Escherichia coli

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