› 8rua

X-ray diffraction
1.71Å resolution

Crystal structure of Rhizobium etli L-asparaginase ReAV C135A mutant

Released:
DOI: 10.2210/pdb8rua/pdb
Model geometry
Fit model/data
PDB entry 8rua coloured by chain, front view.
PDB entry 8rua coloured by chain, side view.
PDB entry 8rua coloured by chain, top view.
Source organism: Rhizobium etli
Primary publication:
Probing the active site of Class 3 L-asparaginase by mutagenesis. I. Tinkering with the zinc coordination site of ReAV.
Pokrywka K, Grzechowiak M, Sliwiak J, Worsztynowicz P, Loch JI, Ruszkowski M, Gilski M, Jaskolski M
OpenAccess logo Front Chem 12 1381032 (2024)
PMID: 38638878

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173668 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-asparaginase II protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 373 amino acids
Theoretical weight: 39.52 KDa
Source organism: Rhizobium etli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2K0Z2 (Residues: 1-367; Coverage: 100%)
Gene names: RHE_PE00350, ansA
Sequence domains: L-asparaginase II

Ligands and Environments

5 bound ligands:
Ligand TRS 1 x TRS
Ligand PEG 3 x PEG
Ligand GOL 9 x GOL
Ligand EDO 8 x EDO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P212121
Unit cell:
a: 78.064Å b: 91.178Å c: 106.049Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.224
Expression system: Escherichia coli

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