8sai

Electron Microscopy
3.27Å resolution

Cryo-EM structure of GPR34-Gi complex

Released:

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-230945 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Probable G-protein coupled receptor 34 Chain: A
Molecule details ›
Chain: A
Length: 466 amino acids
Theoretical weight: 53.32 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UPC5 (Residues: 38-373; Coverage: 88%)
Gene name: GPR34
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: D
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: E
scFv16 Chain: S

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.27Å
Relevant EMDB volumes: EMD-40270
Expression system: Spodoptera frugiperda