› 8smd

X-ray diffraction
2.1Å resolution

Structure of Clostridium botulinum prophage Tad1 in complex with 1''-3' gcADPR

Released:
DOI: 10.2210/pdb8smd/pdb
Model geometry
Fit model/data
PDB entry 8smd coloured by chain, front view.
PDB entry 8smd coloured by chain, side view.
PDB entry 8smd coloured by chain, top view.
Source organism: Clostridium botulinum
Primary publication:
Phages overcome bacterial immunity via diverse anti-defence proteins.
Yirmiya E, Leavitt A, Lu A, Ragucci AE, Avraham C, Osterman I, Garb J, Antine SP, Mooney SE, Hobbs SJ, Kranzusch PJ, Amitai G, Sorek R
Nature (2023)
PMID: 37992756

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-221707 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thoeris anti-defense 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 125 amino acids
Theoretical weight: 14.33 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DW58 (Residues: 1-124; Coverage: 100%)
Gene names: ACP51_06460, tad1

Ligands and Environments

1 bound ligand:
Ligand OJC 2 x OJC
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P213
Unit cell:
a: 95.969Å b: 95.969Å c: 95.969Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.236
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Structural basis of Gabija anti-phage defence and viral immune evasion.
Antine et al. (2024)
1 more