› 8spk

X-ray diffraction
1.6Å resolution

Crystal structure of Antarctic PET-degrading enzyme

Released:
DOI: 10.2210/pdb8spk/pdb
PDB entry 8spk coloured by chain, front view.
PDB entry 8spk coloured by chain, side view.
PDB entry 8spk coloured by chain, top view.
Source organism: Moraxella
Primary publication:
Engineering the catalytic activity of an Antarctic PET-degrading enzyme by loop exchange.
Blázquez-Sánchez P, Vargas JA, Furtado AA, Griñen A, Leonardo DA, Sculaccio SA, Pereira HD, Sonnendecker C, Zimmermann W, Díez B, Garratt RC, Ramírez-Sarmiento CA
Protein Sci e4757 (2023)
PMID: 37574805

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-227139 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 282 amino acids
Theoretical weight: 30.94 KDa
Source organism: Moraxella
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19833 (Residues: 59-319; Coverage: 82%)
Gene names: L1, lip1

Ligands and Environments

1 bound ligand:
Ligand MLI 2 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS SIRIUS BEAMLINE MANACA
Spacegroup: P213
Unit cell:
a: 131.18Å b: 131.18Å c: 131.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.153 0.18
Expression system: Escherichia coli BL21(DE3)

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Citations

1 citations in other articles

Engineering the catalytic activity of an Antarctic PET-degrading enzyme by loop exchange.
Blázquez-Sánchez et al. (2023)