› 8swc

X-ray diffraction
2.68Å resolution

RNase H complex with ASO (OOO) and RNA

Released:
DOI: 10.2210/pdb8swc/pdb
Model geometry
Fit model/data
PDB entry 8swc coloured by chain, front view.
PDB entry 8swc coloured by chain, side view.
PDB entry 8swc coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Cho Y-J, Iwamoto N

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-270762 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (3 distinct):
Ribonuclease H1 Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 33.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60930 (Residues: 1-286; Coverage: 100%)
Gene names: RNASEH1, RNH1
Sequence domains:
PO ASO Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
RNA (5'-R(*UP*GP*GP*CP*GP*AP*GP*UP*GP*GP*GP*UP*GP*AP*GP*UP*GP*AP*GP*G)-3') Chain: B

Ligands and Environments

3 bound ligands:
Ligand PO4 2 x PO4
Ligand CA 3 x CA
Ligand SO4 1 x SO4
5 modified residues:
Ligand OMC 4 x OMC
Ligand C5L 2 x C5L
Ligand T39 1 x T39
Ligand A2M 2 x A2M
Ligand OMG 1 x OMG

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P3121
Unit cell:
a: 100.617Å b: 100.617Å c: 82.092Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.192 0.22
Expression systems:
  • Escherichia coli
  • Not provided

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