› 8t23

Electron Microscopy
3.87Å resolution

Cryo-EM structure of the RBD-ACE2 interface of the SARS-CoV-2 trimeric spike protein bound to ACE2 receptor after local refinement at upRBD conformation

Released:
DOI: 10.2210/pdb8t23/pdb
PDB entry 8t23 coloured by chain, front view.
PDB entry 8t23 coloured by chain, side view.
PDB entry 8t23 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of the American mink ACE2 binding by Y453F trimeric spike glycoproteins of SARS-CoV-2.
Ahn H, Calderon BM, Fan X, Gao Y, Horgan NL, Jiang N, Blohm DS, Hossain J, Rayyan NWK, Osman SH, Lin X, Currier M, Steel J, Wentworth DE, Zhou B, Liang B
J Med Virol 95 e29163 (2023)
PMID: 37842796
Related structures: EMD-40979

Function and Biology Details

Reaction catalysed: 
Angiotensin II + H(2)O = angiotensin-(1-7) + L-phenylalanine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-269579 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Angiotensin-converting enzyme Chain: A
Molecule details ›
Chain: A
Length: 771 amino acids
Theoretical weight: 89.38 KDa
Source organism: Neogale vison
Expression system: Homo sapiens
UniProt:
  • Canonical: A0A7T0Q2W2 (Residues: 1-739; Coverage: 92%)
Sequence domains:
Spike protein S1 Chain: B
Molecule details ›
Chain: B
Length: 208 amino acids
Theoretical weight: 23.37 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DTC2 (Residues: 321-528; Coverage: 17%)
Gene names: 2, S
Sequence domains: Betacoronavirus spike glycoprotein S1, receptor binding

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.87Å
Relevant EMDB volumes: EMD-40979
Expression system: Homo sapiens

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