› 8tnh

Electron Microscopy
3.32Å resolution

Cryo-EM structure of HIV-1 Env BG505 DS-SOSIP in complex with broadly neutralizing llama nanobody G36 targeting the CD4-binding site

Released:
DOI: 10.2210/pdb8tnh/pdb
PDB entry 8tnh coloured by chain, front view.
PDB entry 8tnh coloured by chain, side view.
PDB entry 8tnh coloured by chain, top view.
Source organisms:
Primary publication:
Ultrapotent Broadly Neutralizing Human-llama Bispecific Antibodies against HIV-1.
Xu J, Zhou T, McKee K, Zhang B, Liu C, Nazzari AF, Pegu A, Shen CH, Becker JE, Bender MF, Chan P, Changela A, Chaudhary R, Chen X, Einav T, Kwon YD, Lin BC, Louder MK, Merriam JS, Morano NC, O'Dell S, Olia AS, Rawi R, Roark RS, Stephens T, Teng IT, Tourtellott-Fogt E, Wang S, Yang ES, Shapiro L, Tsybovsky Y, Doria-Rose NA, Casellas R, Kwong PD
Adv Sci (Weinh) 11 e2309268 (2024)
PMID: 38704686
Related structures: EMD-41416

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-244256 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (6 distinct):
Envelope glycoprotein gp160 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 481 amino acids
Theoretical weight: 54.09 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S6 (Residues: 30-505; Coverage: 55%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Transmembrane protein gp41 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 153 amino acids
Theoretical weight: 17.15 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S6 (Residues: 509-661; Coverage: 18%)
Gene name: env
Sequence domains: Retroviral envelope protein
CD4-binding site nanobody G36 Chains: H, I, J

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand NAG 27 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.32Å
Relevant EMDB volumes: EMD-41416
Expression system: Homo sapiens

Quick links

Citations

1 citations in other articles

Ultrapotent Broadly Neutralizing Human-llama Bispecific Antibodies against HIV-1.
Xu et al. (2024)