› 8tpw

Electron Microscopy
3.46Å resolution

Crosslinked 6-deoxyerythronolide B synthase (DEBS) Module 3 in complex with antibody fragment 1B2: cis-oriented 1B2 and ACP

Released:
DOI: 10.2210/pdb8tpw/pdb
PDB entry 8tpw coloured by chain, front view.
PDB entry 8tpw coloured by chain, side view.
PDB entry 8tpw coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for intermodular communication in assembly-line polyketide biosynthesis.
Cogan DP, Soohoo AM, Chen M, Liu Y, Brodsky KL, Khosla C
Nat Chem Biol (2024)
PMID: 39179672
Related structures: EMD-41495

Function and Biology Details

Reaction catalysed: 
Propanoyl-CoA + 6 (2S)-methylmalonyl-CoA + 6 NADPH = 6-deoxyerythronolide B + 7 CoA + 6 CO(2) + H(2)O + 6 NADP(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-252077 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Erythronolide synthase EryA2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1766 amino acids
Theoretical weight: 186.49 KDa
Source organism: Saccharopolyspora erythraea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03132 (Residues: 2-1465; Coverage: 41%)
Gene name: eryA
Sequence domains:
Antibody Fragment 1B2, Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 249 amino acids
Theoretical weight: 26.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Antibody Fragment 1B2, Light Chain Chain: L

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 4HH 3 x 4HH

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.46Å
Relevant EMDB volumes: EMD-41495
Expression system: Escherichia coli BL21(DE3)

Quick links