› 8ttp

X-ray diffraction
1.43Å resolution

Crystal structure of class C beta-lactamase from Escherichia coli in complex with avibactam

Released:
DOI: 10.2210/pdb8ttp/pdb
Model geometry
Fit model/data
PDB entry 8ttp coloured by chain, front view.
PDB entry 8ttp coloured by chain, side view.
PDB entry 8ttp coloured by chain, top view.
Source organism: Escherichia coli A35218R
Entry authors: Chang C, Maltseva N, Endres M, Joachimiak A, Center for Structural Biology of Infectious Diseases (CSBID)

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-269253 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 361 amino acids
Theoretical weight: 39.86 KDa
Source organism: Escherichia coli A35218R
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A377KDY3 (Residues: 30-388; Coverage: 100%)
Gene names: NCTC9075_06171, ampC_2
Sequence domains: Beta-lactamase

Ligands and Environments

4 bound ligands:
Ligand NXL 2 x NXL
Ligand EDO 10 x EDO
Ligand PEG 2 x PEG
Ligand IPA 2 x IPA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2
Unit cell:
a: 155.614Å b: 46.457Å c: 109.126Å
α: 90° β: 116.58° γ: 90°
R-values:
R R work R free
0.161 0.16 0.189
Expression system: Escherichia coli BL21(DE3)

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