› 8uzf

X-ray diffraction
3.28Å resolution

Crystal structure of chimeric RaTG13 RBD complexed with chimeric mouse ACE2

Released:
DOI: 10.2210/pdb8uzf/pdb
Model geometry
Fit model/data
PDB entry 8uzf coloured by chain, front view.
PDB entry 8uzf coloured by chain, side view.
PDB entry 8uzf coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for mouse receptor recognition by bat SARS2-like coronaviruses.
Zhang W, Shi K, Hsueh FC, Mendoza A, Ye G, Huang L, Perlman S, Aihara H, Li F
Proc Natl Acad Sci U S A 121 e2322600121 (2024)
PMID: 39083418

Function and Biology Details

Reaction catalysed: 
Angiotensin II + H(2)O = angiotensin-(1-7) + L-phenylalanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274377 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Processed angiotensin-converting enzyme 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 597 amino acids
Theoretical weight: 69.08 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8R0I0 (Residues: 19-58, 78-102, 322-367; Coverage: 14%)
  • Canonical: Q9BYF1 (Residues: 59-77, 103-321, 368-615; Coverage: 62%)
Gene names: ACE2, Ace2, UNQ868/PRO1885
Sequence domains: Angiotensin-converting enzyme
Receptor binding domain Chains: E, F
Molecule details ›
Chains: E, F
Length: 217 amino acids
Theoretical weight: 24.47 KDa
Source organism: Bat coronavirus RaTG13
Expression system: Spodoptera frugiperda

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
4 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 2 x CL
Ligand EDO 4 x EDO
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 78.556Å b: 119.004Å c: 109.075Å
α: 90° β: 95.92° γ: 90°
R-values:
R R work R free
0.226 0.223 0.28
Expression system: Spodoptera frugiperda

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