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› 8v11

X-ray diffraction
3.95Å resolution

Structure of a Saccharomyces cerevisiae Ipl1 peptide Bound to dwarf Ndc80 complex

Released:
DOI: 10.2210/pdb8v11/pdb
Model geometry
Fit model/data
PDB entry 8v11 coloured by chain, front view.
PDB entry 8v11 coloured by chain, side view.
PDB entry 8v11 coloured by chain, top view.
Source organisms:
Primary publication:
A communication hub for phosphoregulation of kinetochore-microtubule attachment.
Zahm JA, Harrison SC
Curr Biol (2024)
PMID: 38776904

Function and Biology Details

Reaction catalysed: 
(1) L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+).
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-273573 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Kinetochore protein NDC80 Chains: A, E
Molecule details ›
Chains: A, E
Length: 272 amino acids
Theoretical weight: 32.46 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40460 (Residues: 114-684; Coverage: 39%)
Gene names: HEC1, NDC80, TID3, YIL144W
Sequence domains:
Aurora kinase; Kinetochore protein NUF2 Chains: B, F
Molecule details ›
Chains: B, F
Length: 227 amino acids
Theoretical weight: 26.55 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38991 (Residues: 26-59, 192-192; Coverage: 8%)
  • Canonical: P33895 (Residues: 2-157, 159-172; Coverage: 37%)
Gene names: IPL1, NUF2, YOL069W, YPL209C
Sequence domains: Nuf2 family
Kinetochore protein SPC24 Chains: C, G
Kinetochore protein SPC25 Chains: D, H

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 2.0.1
Spacegroup: P43212
Unit cell:
a: 114.757Å b: 114.757Å c: 423.841Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.27 0.267 0.327
Expression system: Escherichia coli BL21(DE3)

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