› 8wp2

Electron Microscopy
3.3Å resolution

MapSPARTA tetramer bound with guide-target

Released:
DOI: 10.2210/pdb8wp2/pdb
PDB entry 8wp2 coloured by chain, front view.
PDB entry 8wp2 coloured by chain, side view.
PDB entry 8wp2 coloured by chain, top view.
Source organism: Maribacter polysiphoniae
Entry authors: Huang PP, Li ZX, Guo LJ, Xiao YB, Chen MR
Related structures: EMD-37708

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-283710 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (4 distinct):
Piwi domain-containing protein Chains: A, C, I, K
Molecule details ›
Chains: A, C, I, K
Length: 507 amino acids
Theoretical weight: 58.09 KDa
Source organism: Maribacter polysiphoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A316E3U6 (Residues: 1-507; Coverage: 100%)
Gene name: LX92_01809
TIR domain-containing protein Chains: B, D, J, L
Molecule details ›
Chains: B, D, J, L
Length: 452 amino acids
Theoretical weight: 53.27 KDa
Source organism: Maribacter polysiphoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A316E683 (Residues: 1-452; Coverage: 100%)
Gene name: LX92_01810
Sequence domains: TIR domain
DNA (25-MER) Chains: F, H, N, P
RNA (5'-R(P*UP*GP*AP*CP*GP*GP*CP*UP*CP*UP*AP*AP*UP*CP*UP*AP*UP*UP*AP*GP*U)-3') Chains: E, G, M, O

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.3Å
Relevant EMDB volumes: EMD-37708
Expression system: Escherichia coli BL21(DE3)

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