› 8xi2

Electron Microscopy
2.6Å resolution

Cryo-EM structure of the Chlamydomonas C* complex

Released:
DOI: 10.2210/pdb8xi2/pdb
PDB entry 8xi2 coloured by chain, front view.
PDB entry 8xi2 coloured by chain, side view.
PDB entry 8xi2 coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Structure of a step II catalytically activated spliceosome from Chlamydomonas reinhardtii.
Lu Y, Liang K, Zhan X
EMBO J (2024)
PMID: 39415054
Related structures: EMD-38362

Function and Biology Details

Reactions catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 34-mer (preferred)
PDBe Complex ID:
PDB-CPX-300461 (preferred)
Entry contents:
26 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (31 distinct):
MPN domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 2398 amino acids
Theoretical weight: 278.54 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A0A2K3E5Q8 (Residues: 1-2398; Coverage: 100%)
Gene name: CHLRE_01g016050v5
Sequence domains:
Elongation factor Tu, chloroplastic Chain: C
Molecule details ›
Chain: C
Length: 989 amino acids
Theoretical weight: 109.37 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8JCA8 (Residues: 1-989; Coverage: 100%)
Gene name: CHLRE_17g737250v5
Sequence domains:
U5-40K Chain: E
Pre-mRNA-processing factor 19 Chains: q, r, s, t
Small nuclear ribonucleoprotein Sm D3 Chain: a
Small nuclear ribonucleoprotein G Chain: g
Small nuclear ribonucleoprotein E Chain: e
Sm protein F Chain: f
Small nuclear ribonucleoprotein Sm D2 Chain: d
Small nuclear ribonucleoprotein Sm D1 Chain: c
Sm protein B Chain: b
Syf1 Chain: I
Crooked neck protein Chain: J
Cwf15/Cwc15 cell cycle control protein Chain: P
peptidylprolyl isomerase Chain: M
PLRG1 Chain: T
Rbm22 Chain: O
G10 protein Chain: N
SKI-interacting protein SKIP SNW domain-containing protein Chain: R
Peptidyl-prolyl cis-trans isomerase Chain: S
Pre-mRNA-processing factor 17 Chain: W
Cdc5L Chain: L
Pre-mRNA-splicing factor SPF27 Chain: K
CWF21 domain-containing protein Chain: U
MI domain-containing protein Chain: V
Intron-binding protein aquarius Chain: Q
U5 snRNA Chain: B
U6 snRNA Chain: F
U2 snRNA Chain: H
RNA (5'-R(P*CP*CP*GP*AP*AP*CP*G)-3') Chain: 5
RNA (173-mer) Chain: 3

Ligands and Environments

3 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 7 x MG
Ligand ZN 3 x ZN
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.6Å
Relevant EMDB volumes: EMD-38362

Quick links