› 8xku

Electron Microscopy
3.2Å resolution

Cryo-EM structure of the Ycf2-FtsHi motor complex from Arabidopsis in ATP-bound state

Released:
DOI: 10.2210/pdb8xku/pdb
PDB entry 8xku coloured by chain, front view.
PDB entry 8xku coloured by chain, side view.
PDB entry 8xku coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural insights into the chloroplast protein import in land plants.
Liang K, Jin Z, Zhan X, Li Y, Xu Q, Xie Y, Yang Y, Wang S, Wu J, Yan Z
Cell (2024)
PMID: 39197452
Related structures: EMD-38425

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = oxaloacetate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptadecamer (preferred)
PDBe Complex ID:
PDB-CPX-281229 (preferred)
Entry contents:
16 distinct polypeptide molecules
Macromolecules (16 distinct):
Probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 855 amino acids
Theoretical weight: 96.98 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: F4KF14 (Residues: 1-855; Coverage: 100%)
Gene names: At5g64580, EMB3144, FTSHI4, MUB3.10
Sequence domains:
ATP-dependent zinc metalloprotease FTSH 12, chloroplastic Chain: B
Molecule details ›
Chain: B
Length: 1008 amino acids
Theoretical weight: 115.27 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q9SAJ3 (Residues: 1-1008; Coverage: 100%)
Gene names: At1g79560, FTSH12, T8K14.2
Sequence domains:
Probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic Chain: C
Protein Ycf2 Chain: D
Probable inactive ATP-dependent zinc metalloprotease FTSHI 1, chloroplastic Chain: E
Probable inactive ATP-dependent zinc metalloprotease FTSHI 2, chloroplastic Chain: F
Protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase Chain: G
Embryo defective 1923 Chain: H
Malate dehydrogenase, chloroplastic Chains: I, J
Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B Chain: K
UNK Chain: L
UNK Chain: M
UNK Chain: N
UNK Chain: O
UNK Chain: P
AtTam37 zinc finger domain-containing protein Chain: R

Ligands and Environments

4 bound ligands:
Ligand MG 2 x MG
Ligand ATP 2 x ATP
Ligand ZN 3 x ZN
Ligand PX2 1 x PX2
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.2Å
Relevant EMDB volumes: EMD-38425

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