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› 8y03

X-ray diffraction
2.93Å resolution

Crystal structure of LbCas12a-crRNA complex

Released:
DOI: 10.2210/pdb8y03/pdb
Model geometry
Fit model/data
PDB entry 8y03 coloured by chain, front view.
PDB entry 8y03 coloured by chain, side view.
PDB entry 8y03 coloured by chain, top view.
Source organism: Lachnospiraceae bacterium ND2006
Primary publication:
DNA target binding-induced pre-crRNA processing in type II and V CRISPR-Cas systems.
Chen J, Lin X, Xiang W, Chen Y, Zhao Y, Huang L, Liu L
Nucleic Acids Res (2024)
PMID: 39676682

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-286339 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Cpf1 Chain: A
Molecule details ›
Chain: A
Length: 1228 amino acids
Theoretical weight: 143.89 KDa
Source organism: Lachnospiraceae bacterium ND2006
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A182DWE3 (Residues: 3-1228; Coverage: 100%)
Sequence domains:
RNA (41-MER) Chain: G
Molecule details ›
Chain: G
Length: 40 nucleotides
Theoretical weight: 12.8 KDa
Source organism: Lachnospiraceae bacterium ND2006
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: C2
Unit cell:
a: 168.259Å b: 83.221Å c: 124.304Å
α: 90° β: 106.208° γ: 90°
R-values:
R R work R free
0.27 0.269 0.289
Expression system: Escherichia coli BL21(DE3)

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