› 9ant

X-ray diffraction
2.4Å resolution

ANTENNAPEDIA HOMEODOMAIN-DNA COMPLEX

Released:
DOI: 10.2210/pdb9ant/pdb
PDB entry 9ant coloured by chain, front view.
PDB entry 9ant coloured by chain, side view.
PDB entry 9ant coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Comparison of X-ray and NMR structures for the Antennapedia homeodomain-DNA complex.
Fraenkel E, Pabo CO
Nat Struct Biol 5 692-7 (1998)
PMID: 9699632

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122634 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homeotic protein antennapedia Chains: A, B
Molecule details ›
Chains: A, B
Length: 62 amino acids
Theoretical weight: 8.09 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P02833 (Residues: 295-356; Coverage: 16%)
Gene names: Antp, CG1028
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
DNA (5'-D(*AP*GP*AP*AP*AP*GP*CP*CP*AP*TP*TP*AP*GP*AP*G)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 15 nucleotides
Theoretical weight: 4.65 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
DNA (5'-D(*TP*CP*TP*CP*TP*AP*AP*TP*GP*GP*CP*TP*TP*TP*C)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 15 nucleotides
Theoretical weight: 4.53 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P2221
Unit cell:
a: 61.05Å b: 77.75Å c: 94.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.239
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Theory, practice, and applications of paramagnetic relaxation enhancement for the characterization of transient low-population states of biological macromolecules and their complexes.
Clore et al. (2009)
4 more

32 mentions without citation

An overview of the structures of protein-DNA complexes.
Luscombe et al. (2000)
31 more