› 9avl

Electron Microscopy
3.8Å resolution

Structure of human calcium-sensing receptor in complex with Gi3 protein in nanodiscs

Released:
DOI: 10.2210/pdb9avl/pdb
PDB entry 9avl coloured by chain, front view.
PDB entry 9avl coloured by chain, side view.
PDB entry 9avl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Promiscuous G-protein activation by the calcium-sensing receptor.
Zuo H, Park J, Frangaj A, Ye J, Lu G, Manning JJ, Asher WB, Lu Z, Hu GB, Wang L, Mendez J, Eng E, Zhang Z, Lin X, Grassucci R, Hendrickson WA, Clarke OB, Javitch JA, Conigrave AD, Fan QR
Nature (2024)
PMID: 38632411
Related structures: EMD-43908

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-272457 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Extracellular calcium-sensing receptor Chains: Q, R
Molecule details ›
Chains: Q, R
Length: 911 amino acids
Theoretical weight: 102.86 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P41180 (Residues: 1-19, 20-903; Coverage: 84%)
Gene names: CASR, GPRC2A, PCAR1
Sequence domains:
Guanine nucleotide-binding protein G(i) subunit alpha-3 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.58 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P08754 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI3
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2 Chain: B
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
7 bound ligands:
Ligand NAG 4 x NAG
Ligand TCR 2 x TCR
Ligand PO4 2 x PO4
Ligand CA 6 x CA
Ligand 9IG 2 x 9IG
Ligand Y01 1 x Y01
Ligand A1AF7 1 x A1AF7
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.8Å
Relevant EMDB volumes: EMD-43908
Expression system: Homo sapiens

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