› 9b85

Electron Microscopy
3.47Å resolution

Cryo-EM structure of human dynactin complex bound to Chlamydia effector Dre1

Released:
DOI: 10.2210/pdb9b85/pdb
PDB entry 9b85 coloured by chain, front view.
PDB entry 9b85 coloured by chain, side view.
PDB entry 9b85 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The Chlamydia effector Dre1 binds dynactin to reposition host organelles during infection.
Sherry J, Pawar KI, Dolat L, Smith E, Chang IC, Pha K, Kaake R, Swaney DL, Herrera C, McMahon E, Bastidas RJ, Johnson JR, Valdivia RH, Krogan NJ, Elwell CA, Verba K, Engel JN
Cell Rep 44 115509 (2025)
PMID: 40186871
Related structures: EMD-44333

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero nonadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-292729 (preferred)
Entry contents:
9 distinct polypeptide molecules
Macromolecules (9 distinct):
Alpha-centractin Chains: A, B, C, D, E, F, G, I
Molecule details ›
Chains: A, B, C, D, E, F, G, I
Length: 376 amino acids
Theoretical weight: 42.67 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61163 (Residues: 1-376; Coverage: 100%)
Gene names: ACTR1A, CTRN1
Sequence domains: Actin
Actin, cytoplasmic 1 Chain: H
Molecule details ›
Chain: H
Length: 375 amino acids
Theoretical weight: 41.78 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P60709 (Residues: 1-375; Coverage: 100%)
Gene name: ACTB
Sequence domains: Actin
Actin-related protein 10 Chain: J
Dynactin subunit 4 Chain: K
Dynactin subunit 5 Chain: L
Dynactin subunit 6 Chain: M
F-actin-capping protein subunit alpha-1 Chain: N
F-actin-capping protein subunit beta Chain: O
Dynactin subunit 2 Chains: P, Q, p, q

Ligands and Environments

3 bound ligands:
Ligand ADP 8 x ADP
Ligand ANP 1 x ANP
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.47Å
Relevant EMDB volumes: EMD-44333

Quick links