› 9b8r

Electron Microscopy
3.5Å resolution

Cryo-EM structure of S. cerevisiae PolE-core-DNA

Released:
DOI: 10.2210/pdb9b8r/pdb
PDB entry 9b8r coloured by chain, front view.
PDB entry 9b8r coloured by chain, side view.
PDB entry 9b8r coloured by chain, top view.
Source organisms:
Primary publication:
Mechanism of PCNA loading by Ctf18-RFC for leading-strand DNA synthesis.
Yuan Z, Georgescu R, Yao NY, Yurieva O, O'Donnell ME, Li H
Science 385 eadk5901 (2024)
PMID: 39088616
Related structures: EMD-44356

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-281333 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase epsilon catalytic subunit A Chain: E
Molecule details ›
Chain: E
Length: 1160 amino acids
Theoretical weight: 133.9 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P21951 (Residues: 27-1186; Coverage: 52%)
Gene names: DUN2, N0825, POL2, YNL262W
Sequence domains:
Primer DNA Chain: P
Molecule details ›
Chain: P
Length: 10 nucleotides
Theoretical weight: 3.03 KDa
Source organism: DNA molecule
Expression system: Not provided
Template DNA Chain: T

Ligands and Environments

1 bound ligand:
Ligand SF4 1 x SF4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.5Å
Relevant EMDB volumes: EMD-44356
Expression systems:
  • Saccharomyces cerevisiae
  • Not provided

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