› 9b8t

Electron Microscopy
2.95Å resolution

Human polymerase epsilon bound to PCNA and DNA in the nucleotide bound state

Released:
DOI: 10.2210/pdb9b8t/pdb
PDB entry 9b8t coloured by chain, front view.
PDB entry 9b8t coloured by chain, side view.
PDB entry 9b8t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of the human leading strand Polε-PCNA holoenzyme.
He Q, Wang F, Yao NY, O'Donnell ME, Li H
Nat Commun 15 7847 (2024)
PMID: 39245668
Related structures: EMD-44358

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-281334 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase epsilon catalytic subunit A Chain: A
Molecule details ›
Chain: A
Length: 2286 amino acids
Theoretical weight: 261.78 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q07864 (Residues: 1-2286; Coverage: 100%)
Gene names: POLE, POLE1
Sequence domains:
Proliferating cell nuclear antigen Chains: B, C, D
Molecule details ›
Chains: B, C, D
Length: 261 amino acids
Theoretical weight: 28.8 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P12004 (Residues: 1-261; Coverage: 100%)
Gene name: PCNA
Sequence domains:
Primer DNA Chain: P
Template DNA Chain: T

Ligands and Environments

3 bound ligands:
Ligand TTP 1 x TTP
Ligand MG 1 x MG
Ligand SF4 1 x SF4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.95Å
Relevant EMDB volumes: EMD-44358
Expression system: Insect cell expression vector pTIE1

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