› 9ex5

X-ray diffraction
2.01Å resolution

Crystal structure of Yeast Clathrin Heavy Chain N-terminal domain bound to Epsin-5 peptide (LIDL)

Released:
DOI: 10.2210/pdb9ex5/pdb
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PDB entry 9ex5 coloured by chain, front view.
PDB entry 9ex5 coloured by chain, side view.
PDB entry 9ex5 coloured by chain, top view.
Source organisms:
Primary publication:
Subtleties in Clathrin heavy chain binding boxes provide selectivity among adaptor proteins of budding yeast.
Defelipe LA, Veith K, Burastero O, Kupriianova T, Bento I, Skruzny M, Kölbel K, Uetrecht C, Thuenauer R, García-Alai MM
Nat Commun 15 9655 (2024)
PMID: 39511183

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-281565 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Clathrin heavy chain Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 373 amino acids
Theoretical weight: 40.98 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22137 (Residues: 1-369; Coverage: 22%)
Gene names: CHC1, YGL206C
Sequence domains:
Epsin-5 Chains: D, E, F, G, H, I, J, L, M
Molecule details ›
Chains: D, E, F, G, H, I, J, L, M
Length: 9 amino acids
Theoretical weight: 1.03 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: Q03769 (Residues: 290-298; Coverage: 2%)
Gene names: ENT5, YDR153C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: C2221
Unit cell:
a: 76.995Å b: 133.449Å c: 285.527Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.215
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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