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› 9eyy

Electron Microscopy
3.3Å resolution

Poliovirus type 1 (strain Mahoney) native conformation stabilised virus-like particle (PV1 SC6b) from a yeast expression system.

Released:
DOI: 10.2210/pdb9eyy/pdb
PDB entry 9eyy coloured by chain, front view.
PDB entry 9eyy coloured by chain, side view.
PDB entry 9eyy coloured by chain, top view.
Source organism: Human poliovirus 1 Mahoney
Primary publication:
Recombinant expression systems for production of stabilised virus-like particles as next-generation polio vaccines.
Sherry L, Bahar MW, Porta C, Fox H, Grehan K, Nasta V, Duyvesteyn HME, De Colibus L, Marsian J, Murdoch I, Ponndorf D, Kim SR, Shah S, Carlyle S, Swanson JJ, Matthews S, Nicol C, Lomonossoff GP, Macadam AJ, Fry EE, Stuart DI, Stonehouse NJ, Rowlands DJ
Nat Commun 16 831 (2025)
PMID: 39827284
Related structures: EMD-50064

Function and Biology Details

Reactions catalysed: 
RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate.
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. Inother picornavirus reactions Glu may be substituted for Gln, and Ser orThr for Gly.
Selective cleavage of Tyr-|-Gly bond in the picornavirus polyprotein.
a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate+ phosphate + H(+).
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 180-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136698 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Capsid protein VP1 Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 33.45 KDa
Source organism: Human poliovirus 1 Mahoney
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P03300 (Residues: 580-881; Coverage: 14%)
Sequence domains: picornavirus capsid protein
Capsid protein VP0 Chain: B
Molecule details ›
Chain: B
Length: 341 amino acids
Theoretical weight: 37.47 KDa
Source organism: Human poliovirus 1 Mahoney
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P03300 (Residues: 1-341; Coverage: 15%)
Sequence domains:
Capsid protein VP3 Chain: C

Ligands and Environments

1 bound ligand:
Ligand PLM 1 x PLM
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.3Å
Relevant EMDB volumes: EMD-50064
Expression system: Komagataella pastoris

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