› 9fju

Electron Microscopy
3.84Å resolution

Structure of the DNase I- and phalloidin-bound pointed end of F-actin (conformer 1)

Released:
DOI: 10.2210/pdb9fju/pdb
PDB entry 9fju coloured by chain, front view.
PDB entry 9fju coloured by chain, side view.
PDB entry 9fju coloured by chain, top view.
Source organisms:
Primary publication:
Phalloidin and DNase I-bound F-actin pointed end structures reveal principles of filament stabilization and disassembly.
Boiero Sanders M, Oosterheert W, Hofnagel O, Bieling P, Raunser S
Nat Commun 15 7969 (2024)
PMID: 39261469
Related structures: EMD-50516

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-271962 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Actin, cytoplasmic 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 374 amino acids
Theoretical weight: 41.66 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P60712 (Residues: 2-375; Coverage: 100%)
Gene name: ACTB
Sequence domains: Actin
Deoxyribonuclease-1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 282 amino acids
Theoretical weight: 31.37 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00639 (Residues: 1-282; Coverage: 100%)
Gene names: DNASE1, DNL1
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Phalloidin Chains: G, H, I, J

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand ADP 4 x ADP
Ligand MG 8 x MG
Ligand PO4 4 x PO4
3 modified residues:
Ligand HIC 4 x HIC
Ligand DTH 4 x DTH
Ligand HYP 4 x HYP

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.84Å
Relevant EMDB volumes: EMD-50516

Quick links